论文题目：Stripe rust fungi hijack a host transcriptional repressor to induce TaSWEET14d for enhanced sugar uptake in wheat
论文作者：Peijing Zheng, Pu Yuan, Nannan Fang, Xiaoxuan Ma, Lihua Jiang, Qingdong Zeng, Dejun Han, Zhensheng Kang, Jie Liu*
论文摘要：
Biotrophic pathogens rely on host plants for carbon acquisition, making plant sugar transporters, such as Sugars Will Eventually be Exported Transporters (SWEETs), key targets in sugar competition between plants and pathogens. In this study, we identified TaSWEET14d as a Puccinia striiformis f. sp. tritici ( Pst )-induced, secretory vesicle-localized SWEET family member that facilitates hexose and sucrose translocation in wheat. Knockout of TaSWEET14d led to enhanced wheat resistance to Pst , while overexpressing TaSWEET14d promoted susceptibility to stripe rust and sugar accumulation in wheat leaves. In addition, wheat transcription factor TaMYB50 contributed to stripe rust resistance by suppressing TaSWEET14d expression through binding directly to the TA-box motif in the promoter region. Furthermore, we found that effector protein Pst15882, required for Pst infection and pathogenicity, interacted with TaMYB50 to prevent this repression, thus revealing a distinctive regulation strategy by Pst to exploit host sugars for fungal invasion.
活体营养型寄生病原菌依赖寄主植物获取碳源，这使得植物糖转运蛋白，如最终将被输出的糖转运蛋白（SWEETs）成为植物与病原菌之间糖分竞争的关键靶点。本研究鉴定出 TaSWEET14d 是一个受小麦条锈菌诱导、定位于分泌囊泡的SWEET家族成员，可以促进小麦中己糖和蔗糖的转运。敲除 TaSWEET14d 增强了小麦对条锈菌的抗性，而过表达 TaSWEET14d 则提高了小麦对条锈菌的感病性并导致叶片中糖分积累。此外，小麦转录因子TaMYB50通过直接结合到 TaSWEET14d 启动子区的TA-box基序来抑制其表达，从而有助于小麦对条锈菌的抗性。更重要的是，该研究发现条锈菌侵染和致病所必需的效应蛋白Pst15882能够与TaMYB50相互作用，阻止了TaMYB50对 TaSWEET14d 的抑制作用，这揭示了条锈菌利用寄主糖分以促进自身侵染的独特调控策略。
论文链接：https://www.science.org/doi/10.1126/sciadv.adv1760