论文题目: Activation of an antifungal pathway in Yersinia pseudotuberculosis by chitin-receptor mediated fungal recognition
论文作者：Yuqi Liu#, Yuxin Zuo#, Changfu Li#, Peishuai Fu, Xinquan He, Zhuo Wang, Yongdong Li, Chuanxing Wan, Yang Wang, Yao Wang, Lingfang Zhu*, Xihui Shen*
论文摘要：Despite the ubiquitous nature of bacterial-fungal interactions (BFIs), it is not fully understood how bacteria detect the presence of a potential fungal competitor to initiate appropriate defense responses. In this study, we show that the enteropathogen Yersinia pseudotuberculosis ( Yptb ) utilizes the two-component system (TCS) histidine kinase RstB to sense chitin as a pathogen-associated molecular pattern (PAMP) for detecting fungi, resulting in the phosphorylation of the downstream response regulator RstA and subsequent activation of the classical type II secretion system (T2SS) and the tight adherence secretion system (TadSS), a major subtype of T2SS. The activation of T2SS and TadSS facilitates the secretion of chitinase TscE, which eliminates fungi and enhances bacterial colonization of the murine gut. These findings not only elucidate the role of T2SS and TscE in antagonizing fungal competitors, but also offer insights into the interkingdom recognition mechanisms between bacteria and fungi.
尽管细菌-真菌相互作用（BFIs）普遍存在，但我们仍未完全理解细菌如何检测到潜在的真菌竞争者并启动相应的防御反应。本研究中，我们发现肠道病原菌耶尔森氏肠炎菌（ Yersinia pseudotuberculosis , Yptb ）通过双组分系统（TCS）中的组氨酸激酶RstB将几丁质作为病原相关分子模式（PAMP）来感知真菌。这一过程导致下游反应调节因子RstA的磷酸化，进而激活经典的II型分泌系统（T2SS）和紧密附着分泌系统（TadSS）。T2SS和TadSS的激活促进了几丁质酶TscE的分泌，该酶能够有效消灭真菌，并增强细菌在小鼠肠道中的定殖能力。这些发现不仅阐明了T2SS和TscE在对抗真菌竞争者中的关键作用，还为细菌与真菌之间的跨界识别机制提供了新的视角和深入的理解。
原文链接：https://www.cell.com/current-biology/abstract/S0960-9822(25)00569-X