论文题目：
The transcription factor Dof32 coordinates salvianolic acid biosynthesis and drought tolerance in Salvia miltiorrhiza
论文作者：
Bingbing Lv#, Qiaoqiao Feng#, Gaige Shao#, Anqi Zuo, Xuejiao Yan, Jingying Liu, Juane Dong*, and Pengda Ma*
论文摘要：
Drought severely impacts plant growth, yet moderate drought stress can stimulate the synthesis of active compounds in medicinal plants. However, the molecular regulators and mechanisms linking drought resistance with the accumulation of active compounds remain poorly understood. In this study, the Dof transcription factor SmDof32 was identified as a key regulator of both salvianolic acid biosynthesis and drought response in Salvia miltiorrhiza . Overexpression of SmDof32 enhanced both medicinal compound accumulation and drought resistance, whereas SmDof32 RNA interference resulted in reduced accumulation of salvianolic acids and diminished drought tolerance. DNA-affinity purification sequencing (DAP-seq) combined with RNA-sequencing (RNA-seq) analysis revealed that SmDof32 directly promotes the expression of lipoxygenase ( SmLOX8 ) and rosmarinic acid synthase ( SmRAS1 ), thereby increasing the synthesis of jasmonic acid (JA) and salvianolic acids. Further analysis of SmLOX8 overexpression and RNAi lines confirmed that SmLOX8 promotes JA synthesis, enhances drought tolerance, and increases salvianolic acid biosynthesis. Moreover, inhibition of JA biosynthesis significantly reduced the positive effects of SmDof32 on salvianolic acid accumulation and drought tolerance. Collectively, these findings suggest that SmDof32- SmLOX8 / SmRAS1 module plays a crucial role in drought response and the accumulation of salvianolic acids, providing genetic targets for breeding S. miltiorrhiza with enhanced medicinal compound content and drought resilience.
茉莉酸（JAs）是关键植物激素，参与次生代谢调控和非生物胁迫响应。在本研究中，作者发现干旱胁迫可诱导丹参中Dof类转录因子SmDof32的表达。SmDof32可同时响应MeJA处理与干旱胁迫，通过激活JA合成基因SmLOX8与丹酚酸合成相关基因SmRAS1，提升内源JA含量、增强抗旱性并促进丹酚酸积累。功能验证显示，SmDof32过表达增强抗旱性和药用成分合成，RNA干扰则表现出相反趋势。DIECA处理实验进一步证实其调控作用依赖于JA信号通路。上游调控分析表明SmMYB97可激活SmDof32的转录，SmJAZ8通过互作抑制该激活作用。SmDof32介于JA信号感知与代谢/胁迫响应之间，构建了“SmMYB97–SmDof32–SmLOX8/SmRAS1”调控模块，整合了JA信号、活性成分积累与抗旱响应。该研究为中药材优质高抗性育种提供了关键分子基础。
原文链接：https://doi.org/10.1093/plphys/kiaf221