论文题目：LEF-6 phosphorylation regulates the binding and trafficking of baculovirus late gene transcripts
论文作者：Xiaoyue Zhang#, Zhihui Zhang, Ziqian Cao, Jianchao Li, Shengqiang Jiang, Hongying Chen*
论文摘要：
The Autographa californica multiple nucleopolyhedrovirus (AcMNPV) LEF-6 has been shown to accelerate the infection cycle of AcMNPV, but its specific function is yet to be defined. In the present study, we identify LEF-6 as an RNA-binding protein that preferentially interacts with viral late transcripts. We demonstrate that the N-terminal half of LEF-6, which contains two typical ribonucleoprotein (RNP) motifs, is responsible for RNA binding, but its C-terminal half is required for the binding specificity to viral RNAs and for fulfilling its function in promoting virus infection. Further investigations reveal that LEF-6 has eight phosphorylated sites, and seven of them are located in the C-terminal half of LEF-6. The phosphorylation of LEF-6, especially the modification
on the key site of S95, plays an important role in the binding and trafficking of viral late transcripts into the cytoplasm in baculovirus infection. These findings suggest that LEF-6 facilitates baculovirus infection through recognition and nuclear export of viral late mRNAs for protein translation.
苜蓿银纹夜蛾核型多角体病毒（AcMNPV）LEF-6可以加速病毒感染周期，但其具体功能尚未确定。在本研究中，我们明确了LEF-6的RNA结合蛋白特性及其优先结合病毒晚期转录物的功能；证明LEF-6的N端部分包含两个典型的核糖核蛋白基序，负责RNA结合，但其C端部分是与病毒RNA结合特异性和促进病毒感染功能所必需的。进一步的研究表明，LEF-6有8个磷酸化位点，其中7个位于LEF-6的C末端。LEF-6的磷酸化，尤其是S95关键位点的磷酸化在病毒晚期转录物结合和核质运输中起着重要作用。这些发现表明，LEF-6通过优先识别和核输出病毒晚期mRNA以利于病毒蛋白质翻译，进而促进杆状病毒感染。
论文链接：https://doi.org/10.1128/jvi.02100-24