论文题目：4-Hydroxy-5-methyl-3(2H)-furanone mediates widespread interkingdom communications between fungi and prokaryotes
论文作者：Xinyu Qian, Yan-Bo Zhao, Heng Zhang,Wenguang Yang, Kai Sun, Songying Ouyang, Xihui Shen, Lei Zhang
论文摘要：Fungal–prokaryotic interactions are widespread in natural and host-associated habitats. These interactions are believed to play crucial roles in ecosystem functioning and health. However, the cell–cell interaction mechanisms between these two types of microorganisms remain largely unknown.We demonstrate that fungi Saccharomyces cerevisiae and Cryptococcus neoformans secrete 4-hydroxy-5-methyl-3(2H)-furanone (MHF), which chemoattracts Pseudomonas aeruginosa via the dCache_1-type ligand-binding domains (LBDs) of the chemoreceptors PctA and TlpQ. We also solved high-resolution structures of PctA-LBD and TlpQ-LBD in complex with MHF. Bioinformatic analyses suggest that the MHF synthase Cff1p is widespread among fungi, and functionally distinct dCache_1-containing transmembrane receptors capable of sensing MHF are prevalent across diverse bacteria and archaea. Functional assays confirm MHF binding enhances enzymatic activities of representative dCache_1-type LBD-containing receptors.Finally, communication network analysis suggests that MHF-mediated fungal–prokaryotic interactions are widespread between members of the fungal phyla Ascomycota and Basidiomycota and the bacterial phyla Pseudomonadota and Bacillota.These findings identify MHF as a broadly utilisedfungal signal mediating extensive interkingdom communications between fungi and prokaryotes, highlighting its potential role in shaping microbial community dynamics.
真菌与原核生物的相互作用广泛存在于自然环境以及宿主相关生境中，这些相互作用被认为对生态系统的功能和健康发挥至关重要的作用。然而，这两种微生物之间的细胞-细胞相互作用机制仍然知之甚少。本研究中，我们发现真菌酿酒酵母和新型隐球菌分泌一种挥发性化合物4-羟基-5-甲基-3(2H)-呋喃酮（MHF），其靶向含dCache_1结构域的趋化受体PctA和TlpQ趋化吸引铜绿假单胞菌。我们解析了与MHF结合的PctA-LBD和TlpQ-LBD的高分辨率结构。生物信息学分析表明，MHF合成酶Cff1p在真菌中广泛存在，并且在不同的细菌和古细菌中普遍存在功能多样的含dCache_1结构域的能够感知MHF信号的跨膜受体。MHF对一些代表性受体的功能调控作用被实验验证。通讯网络分析表明，MHF介导的真菌-原核相互作用在真菌的子囊菌门和担子菌门以及细菌的假单胞菌门和芽孢杆菌门之间广泛存在。综上，本研究确定MHF是一种广泛存在的真菌信号，介导真菌和原核生物之间广泛的界间通信，其在塑造微生物群落动态中的潜在作用有待深入研究。
原文链接：https://link.springer.com/article/10.1186/s13059-026-04138-3